解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
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公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Integrins are major receptors used by cells to interact with extracellular matrices. In this paper, we identify the first ligands for the beta 8 family of integrins, presenting evidence that integrin heterodimers containing the beta 8 subunit mediate interactions of chick sensory neurons with laminin-1, collagen IV, a...
journal_title:Molecular biology of the cell
pub_type: 杂志文章
doi:10.1091/mbc.6.4.419
更新日期:1995-04-01 00:00:00
abstract::Recently, we described the constitutive activation of Mek1 by mutation of its two serine phosphorylation sites. We have now characterized the biochemical properties of these Mek1 mutants and performed microinjection experiments to investigate the effect of an activated Mek on oocyte maturation. Single acidic substitut...
journal_title:Molecular biology of the cell
pub_type: 杂志文章
doi:10.1091/mbc.6.3.237
更新日期:1995-03-01 00:00:00
abstract::Cells have evolved an autoregulatory mechanism to dampen variations in the concentration of tubulin monomer that is available to polymerize into microtubules (MTs), a process that is known as tubulin autoregulation. However, thermodynamic analysis of MT polymerization predicts that the concentration of free tubulin mo...
journal_title:Molecular biology of the cell
pub_type: 杂志文章
doi:10.1091/mbc.5.12.1281
更新日期:1994-12-01 00:00:00
abstract::Precise chromosome transmission in cell division cycle is maintained by a number of genes. The attempt made in the present study was to isolate temperature-sensitive (ts) fission yeast mutants that display high loss rates of minichromosomes at permissive or semipermissive temperature (designated mis). By colony color ...
journal_title:Molecular biology of the cell
pub_type: 杂志文章
doi:10.1091/mbc.5.10.1145
更新日期:1994-10-01 00:00:00
abstract::The yeast vacuolar proton-translocating ATPase (V-ATPase) is a multisubunit complex comprised of peripheral membrane subunits involved in ATP hydrolysis and integral membrane subunits involved in proton pumping. The yeast vma21 mutant was isolated from a screen to identify mutants defective in V-ATPase function. vma21...
journal_title:Molecular biology of the cell
pub_type: 杂志文章
doi:10.1091/mbc.5.9.1039
更新日期:1994-09-01 00:00:00
abstract::Recent evidence has shown that members of the Jak kinase family are activated after IL-6 binds to its receptor complex, leading to a tyrosine phosphorylation of gp130, the IL-6 signal-transducing subunit. The different members of the IL-6 cytokine subfamily induce distinct patterns of Jak-Tyk phosphorylation in differ...
journal_title:Molecular biology of the cell
pub_type: 杂志文章
doi:10.1091/mbc.5.7.819
更新日期:1994-07-01 00:00:00
abstract::When demembranated sperm nuclei are placed in a Xenopus egg extract, they become surrounded by a nuclear envelope and then swell to form morphologically typical pronuclei. Granules ranging from < 1.0 to approximately 3.0 microns in diameter appear within such nuclei. Bell et al. identified four nucleolar proteins in t...
journal_title:Molecular biology of the cell
pub_type: 杂志文章
doi:10.1091/mbc.5.6.633
更新日期:1994-06-01 00:00:00
abstract::Activation of the c-myc proto-oncogene by chromosomal translocation or proviral insertion frequently results in the separation of the c-myc coding region from its normal regulatory elements. Such rearrangements are often accompanied by loss or mutation of c-myc exon 1 sequences. These genetic alterations do not affect...
journal_title:Molecular biology of the cell
pub_type: 杂志文章
doi:10.1091/mbc.5.5.597
更新日期:1994-05-01 00:00:00
abstract::The key regulator of entry into mitosis is the serine/threonine kinase p34cdc2. This kinase is regulated both by association with cyclins and by phosphorylation at several sites. Phosphorylation at Tyr 15 and Thr 14 are believed to inhibit the kinase activity of cdc2. In Schizosaccharomyces pombe, the wee1 (and possib...
journal_title:Molecular biology of the cell
pub_type: 杂志文章
doi:10.1091/mbc.5.3.273
更新日期:1994-03-01 00:00:00
abstract::MPM-2 antigens, a discrete set of phosphoproteins that contain similar phosphoepitopes recognized by the monoclonal antibody MPM-2, are phosphorylated during M-phase induction. Our previous studies suggested that certain MPM-2 antigens are involved in the appearance of maturation-promoting factor (MPF) activity. Becau...
journal_title:Molecular biology of the cell
pub_type: 杂志文章
doi:10.1091/mbc.5.2.135
更新日期:1994-02-01 00:00:00
abstract::The Saccharomyces cerevisiae genes TOR1 and TOR2 were originally identified by mutations that confer resistance to the immunosuppressant rapamycin. TOR2 was previously shown to encode an essential 282-kDa phosphatidylinositol kinase (PI kinase) homologue. The TOR1 gene product is also a large (281 kDa) PI kinase homol...
journal_title:Molecular biology of the cell
pub_type: 杂志文章
doi:10.1091/mbc.5.1.105
更新日期:1994-01-01 00:00:00
abstract::We have identified five complementation groups of peroxisome biogenesis (peb) mutants in Saccharomyces cerevisiae by a positive selection procedure. Three of these contained morphologically recognizable peroxisomes, and two appeared to lack the organelle altogether. The packaging of peroxisomal proteins in these mutan...
journal_title:Molecular biology of the cell
pub_type: 杂志文章
doi:10.1091/mbc.4.12.1351
更新日期:1993-12-01 00:00:00
abstract::To further elucidate the functions of actin in budding yeast and to relate actin structure to specific roles and interactions in vivo, we determined the phenotypes caused by 13 charged-to-alanine mutations isolated previously in the single Saccharomyces cerevisiae actin gene. Defects in actin organization, morphogenes...
journal_title:Molecular biology of the cell
pub_type: 杂志文章
doi:10.1091/mbc.4.12.1277
更新日期:1993-12-01 00:00:00
abstract::Activation of the NADPH oxidase of phagocytic cells requires the action of Rac2 or Rac1, members of the Ras superfamily of GTP-binding proteins. Rac proteins are active when in the GTP-bound form and can be regulated by a variety of proteins that modulate the exchange of GDP for GTP and/or GTP hydrolysis. The p190 Rac...
journal_title:Molecular biology of the cell
pub_type: 杂志文章
doi:10.1091/mbc.4.11.1217
更新日期:1993-11-01 00:00:00
abstract::Microtubules (MTs) in newt mitotic spindles grow faster than MTs in the interphase cytoplasmic microtubule complex (CMTC), yet spindle MTs do not have the long lengths or lifetimes of the CMTC microtubules. Because MTs undergo dynamic instability, it is likely that changes in the durations of growth or shortening are ...
journal_title:Molecular biology of the cell
pub_type: 杂志文章
doi:10.1091/mbc.4.10.1035
更新日期:1993-10-01 00:00:00
abstract::A cell surface proteoglycan, syndecan-1, has been shown to participate in the maintenance of the epithelial cell morphology. A point mutated activated c-Ha-ras gene under the control of the glucocorticoid inducible MMTV-LTR promoter was transfected into the mouse mammary epithelial cell line, NOG-8. The NOG-8 ras cell...
journal_title:Molecular biology of the cell
pub_type: 杂志文章
doi:10.1091/mbc.4.8.849
更新日期:1993-08-01 00:00:00
abstract::Low molecular weight (LMW) GTP-binding proteins are hypothesized to play a role in the vectorial transport of intracellular vesicles. Mutational studies in yeast and subcellular localization in mammalian cells suggest that a family of LMW GTP-binding proteins, termed rab, target intracellular vesicles to their appropr...
journal_title:Molecular biology of the cell
pub_type: 杂志文章
doi:10.1091/mbc.4.7.747
更新日期:1993-07-01 00:00:00
abstract::Many integrin receptors localize to focal contact sites upon binding their ligand. However, unoccupied integrin receptors do not localize to focal contact sites. Because the integrin beta 1 cytoplasmic domain appears to have a focal contact localization signal, there must be a mechanism by which this domain is kept in...
journal_title:Molecular biology of the cell
pub_type: 杂志文章
doi:10.1091/mbc.4.6.593
更新日期:1993-06-01 00:00:00
abstract::Cell adhesion to extracellular matrix molecules such as fibronectin involves complex transmembrane signaling processes. Attachment and spreading of primary fibroblasts can be promoted by interactions of cell surface integrins with RGD-containing fragments of fibronectin, but the further process of focal adhesion and s...
journal_title:Molecular biology of the cell
pub_type: 杂志文章
doi:10.1091/mbc.4.6.605
更新日期:1993-06-01 00:00:00
abstract::Rab proteins are thought to function in the processes by which transport vesicles identify and/or fuse with their respective target membranes. The bulk of these proteins are membrane associated, but a measurable fraction can be found in the cytosol. The cytosolic forms of rab3A, rab11, and Sec4 occur as equimolar comp...
journal_title:Molecular biology of the cell
pub_type: 杂志文章
doi:10.1091/mbc.4.4.425
更新日期:1993-04-01 00:00:00
abstract::Intracellular free calcium ([Ca2+]i) has been proposed to play an important part in the regulation of the cell cycle. Although a number of studies have shown that stimulation of quiescent cells with growth factors causes an immediate rise in [Ca2+]i (Rabinovitch et al., 1986; Vincentini and Villereal, 1986; Hesketh et...
journal_title:Molecular biology of the cell
pub_type: 杂志文章
doi:10.1091/mbc.4.3.293
更新日期:1993-03-01 00:00:00
abstract::Two widely used hepatoma cell lines, mouse BW1J and human HepG2, express gene products characteristic of fetal hepatocytes, including serum albumin, whereas reporter genes driven by the albumin promoter are expressed at very low levels compared with highly differentiated hepatoma cells. We have investigated the low al...
journal_title:Molecular biology of the cell
pub_type: 杂志文章
doi:10.1091/mbc.4.1.59
更新日期:1993-01-01 00:00:00
abstract::In vitro Ca++ activates gelsolin to sever F-actin and form a gelsolin-actin (GA) complex at the+end of F-actin that is not dissociated by ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) but is separated by EGTA+PIP/PIP2. The gelsolin blocks the+end on the actin filament, but the-end of the...
journal_title:Molecular biology of the cell
pub_type: 杂志文章
doi:10.1091/mbc.3.12.1427
更新日期:1992-12-01 00:00:00
abstract::The formation and maintenance of the bipolar mitotic spindle apparatus require a complex and balanced interplay of several mechanisms, including the stabilization and separation of polar microtubules and the action of various microtubule motors. Nonmicrotubule elements are also present throughout the spindle apparatus...
journal_title:Molecular biology of the cell
pub_type: 杂志文章
doi:10.1091/mbc.3.11.1259
更新日期:1992-11-01 00:00:00
abstract::Nuclear factor kappa B (NF-kappa B) modulates the expression of numerous genes via interaction with a specific DNA sequence termed the kappa B site. Its activity is modulated by a cytosolic inhibitor protein termed I kappa B, and its activation occurs in response to a variety of agents in a variety of cell types, most...
journal_title:Molecular biology of the cell
pub_type: 杂志文章
doi:10.1091/mbc.3.10.1131
更新日期:1992-10-01 00:00:00
abstract::We demonstrate an example of signal transduction by an integrin and have begun to define the pathway through which this signaling is achieved. We constructed a stably transfected derivative of 293 cells (ATCC 1573) that expresses the platelet integrin GPIIbIIIa (alpha IIb beta 3). This cell line, clone B, adheres to a...
journal_title:Molecular biology of the cell
pub_type: 杂志文章
doi:10.1091/mbc.3.9.989
更新日期:1992-09-01 00:00:00
abstract::The cdc25 tyrosine phosphatase is known to activate cdc2 kinase in the G2/M transition by dephosphorylation of tyrosine 15. To determine how entry into M-phase in eukaryotic cells is controlled, we have investigated the regulation of the cdc25 protein in Xenopus eggs and oocytes. Two closely related Xenopus cdc25 gene...
journal_title:Molecular biology of the cell
pub_type: 杂志文章
doi:10.1091/mbc.3.8.927
更新日期:1992-08-01 00:00:00
abstract::The crystalline lens is an attractive system to study the biology of intercellular communication; however, the identity of the structural components of gap junctions in the lens has been controversial. We have cloned a novel member of the connexin family of gap junction proteins, Cx50, and have shown that it is likely...
journal_title:Molecular biology of the cell
pub_type: 杂志文章
doi:10.1091/mbc.3.7.711
更新日期:1992-07-01 00:00:00
abstract::Lactogenic hormones and extracellular matrix (ECM) act synergistically to regulate beta-casein expression in culture. We have developed a functional subpopulation of the mouse mammary epithelial cell strain COMMA-1D (designated CID 9), which expresses high level of beta-casein, forms alveolar-like structures when plat...
journal_title:Molecular biology of the cell
pub_type: 杂志文章
doi:10.1091/mbc.3.6.699
更新日期:1992-06-01 00:00:00
abstract::We have used E1A probes to study the roles of the p34cdc2 kinase and the retinoblastoma tumor susceptibility gene product (pRB) in transforming growth factor beta 1 (TGF beta 1)-mediated growth suppression in mink lung epithelial (Mv1Lu) cells. In agreement with previous reports, we see a decline in p34cdc2 kinase act...
journal_title:Molecular biology of the cell
pub_type: 杂志文章
doi:10.1091/mbc.3.6.655
更新日期:1992-06-01 00:00:00
abstract::The Schizosaccharomyces pombe sts1+ gene, identified by supersensitive mutations to a protein kinase inhibitor, staurosporine, was isolated by complementation by the use of a fission yeast genomic library. Nucleotide sequencing shows that the sts1+ gene encodes a 453 amino acid putative membrane-associated protein tha...
journal_title:Molecular biology of the cell
pub_type: 杂志文章
doi:10.1091/mbc.3.3.263
更新日期:1992-03-01 00:00:00
abstract::Two cDNAs encoding casein kinase-1 have been isolated from a yeast cDNA library and termed CKI1 and CKI2. Each clone encodes a protein of approximately 62,000 Da containing a highly conserved protein kinase domain surrounded by variable amino- and carboxy-terminal domains. The proteins also contain two conserved carbo...
journal_title:Molecular biology of the cell
pub_type: 杂志文章
doi:10.1091/mbc.3.3.275
更新日期:1992-03-01 00:00:00
abstract::Thrombospondin (TSP) was demonstrated to inhibit the growth of bovine aortic endothelial cells, an activity that was not neutralized by antibodies to TSP or by other agents that block TSP-cell interactions but that partially was reversed by a neutralizing antibody to transforming growth factor-beta (TGF-beta). Similar...
journal_title:Molecular biology of the cell
pub_type: 杂志文章
doi:10.1091/mbc.3.2.181
更新日期:1992-02-01 00:00:00
abstract::To determine how the human cdc25 gene product acts to regulate p34cdc2 at the G2 to M transition, we have overproduced the full-length protein (cdc25Hs) as well as several deletion mutants in bacteria as glutathione-S-transferase fusion proteins. The wild-type cdc25Hs gene product was synthesized as an 80-kDa fusion p...
journal_title:Molecular biology of the cell
pub_type: 杂志文章
doi:10.1091/mbc.3.1.73
更新日期:1992-01-01 00:00:00